| Effect of LPA on activation and endocytosis of microglia |
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| DOI： |
| EN KeyWords: LPA microglia FITC-dextran FCM |
| Fund Project:国家自然科学基金项目(81000521，81030021) |
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| EN Abstract: |
| Objective: To observe the effect of lysophosphatidic acid (LPA) on activation and endocytosis of microglia. Methods: BV2 cells in exponential phase were randomly divided into two groups: control group and LPA group. At time points (1 h and 2 h), cells were harvested for later use. FCM was used to detect the phagocytic rate of FITC-dextran and the fluorescence intensity. Results: As compared with control group, the phagocytic rate of FITC-dextran in LPA group was significantly increased at 1 h (P<0.05). At 2 h, the phagocytic rate of FITC-dextran reached the peak in both two groups. At time points (1 h and 2 h), as compared with control group, the fluorescence intensity in LPA group was increased (P<0.01) and the product of phagocytic rate and fluorescence intensity in LPA group was significantly higher than that of control group (P<0.01). Conclusion: LPA can promote activation and endocytosis of microglia in vitro, suggesting the roles of LPA in activation and endocytosis of microglia. |
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